Generator

Part:BBa_J100089:Design

Designed by: Ben Clarkson   Group: Campbell M Lab   (2012-07-18)


J100072+J10063 (LcpxP+LRE, Luciferase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2460
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1351
    Illegal XhoI site found at 208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1300
    Illegal AgeI site found at 3002
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732
    Illegal SapI.rc site found at 2516


Design Notes

Includes coding sequences for Luciferin Regenerating Enzyme and Luciferase


Source

Promoter from E. coli genome, coding sequence from L. cruciata

References